Cofilin Antikörper (N-Term)

Produktnummer ABIN197372

Der Kaninchen Polyklonal anti-Cofilin Antikörper wird verwendet zum Nachweis von Cofilin in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Cofilin Antikörper (N-Term) (ABIN197372)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cofilin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cofilin Antikörper

Bindungsspezifität: AA 1-5, N-Term

Spezifität: This antibody detects endogenous levels of total Cofilin protein.

Aufreinigung: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around amino acids 1~5 ((M-A-S-G-V) derived from Human Cofilin.

Anwendungsinformationen

Applikationshinweise: Western blot (1/500-1/1000). Immunohistochemistry (1/50-1/100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Andere Bezeichnung: Cofilin-1

Hintergrund: Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH -dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18

Gen-ID: 1072

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events