PKC mu Antikörper (Ser738)

Produktnummer ABIN197312

Der Kaninchen Polyklonal anti-PKC mu Antikörper wird verwendet zum Nachweis von PKC mu in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IF.

Kurzübersicht für PKC mu Antikörper (Ser738) (ABIN197312)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC mu Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-PKC mu Antikörper

Bindungsspezifität: Ser738

Spezifität: This antibody detects endogenous levels of total PKD/PKCμ protein.

Aufreinigung: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human PKD/PKCmy around the phosphorylation site of serine 738 (E-K-Sp-F-R).

Anwendungsinformationen

Applikationshinweise: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Andere Bezeichnung: PRKD1

Hintergrund: Protein Kinase C mu, is a ~140 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC mu (also known as Protein Kinase D or PKD) is implicated in the regulation of multiple cellular processes including Golgi organization and membrane transport in epithelial cells. PKC mu is phosphorylated on serine 742 (serine 748 for the mouse sequence) in the activation loop in a PKC-dependent pathway, mainly by PKC eta and PKC epsilon. This is critical for its catalytic activity, substrate phosphorylation and role in activating the ERK1 MAP Kinase signaling cascade.Synonyms: PKC D1, PKC mu, PKD, PKD1, PRKCM, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, nPKC-D1, nPKC-mu

Gen-ID: 5587

NCBI Accession: NP_002733

UniProt: Q15139

Pathways: Myometrial Relaxation and Contraction