Der Kaninchen Polyklonal Anti-PKC mu-Antikörper wurde für WB und IF validiert. Er ist geeignet, PKC mu in Proben von Human, Maus und Ratte zu detektieren.
This antibody detects endogenous levels of total PKD/PKCμ protein.
Aufreinigung
Immunoaffinity Chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human PKD/PKCmy around the phosphorylation site of serine 738 (E-K-Sp-F-R).
PRKD1
Reaktivität: Human
WB, ELISA, IHC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store the antibody (in aliquots) at -20 °C.
Target
PKC mu (PRKD1)
(Protein Kinase D1 (PRKD1))
Andere Bezeichnung
PRKD1
Hintergrund
Protein Kinase C mu, is a ~140 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC mu (also known as Protein Kinase D or PKD) is implicated in the regulation of multiple cellular processes including Golgi organization and membrane transport in epithelial cells. PKC mu is phosphorylated on serine 742 (serine 748 for the mouse sequence) in the activation loop in a PKC-dependent pathway, mainly by PKC eta and PKC epsilon. This is critical for its catalytic activity, substrate phosphorylation and role in activating the ERK1 MAP Kinase signaling cascade.Synonyms: PKC D1, PKC mu, PKD, PKD1, PRKCM, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, nPKC-D1, nPKC-mu