JunD Antikörper (Ser255)

Produktnummer ABIN197142

Der Kaninchen Polyklonal anti-JunD Antikörper (ABIN197142) detektiert spezifisch JunD in WB, IF und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für JunD Antikörper (Ser255) (ABIN197142)

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser JunD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-JunD Antikörper

Bindungsspezifität: Ser255

Spezifität: JunD antibody detects endogenous levels of total JunD protein.

Aufreinigung: Affinity-chromatography using epitope-specific immunogen

Immunogen: Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of serine 255 (G-E-Sp-P-P).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1: 500approx. 1: 1000. Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS( without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu JunD

Target: JunD (JUND) (Jun D Proto-Oncogene (JUND))

Andere Bezeichnung: Jun-D

Hintergrund: JunD is the most broadly expressed member of the Jun family and the AP1 transcription factor complex. It has been found that primary fibroblasts lacking murine JunD displayed p53-dependent growth arrest, upregulated p19(ARF) expression, and premature senescence. In contrast, immortalized cell lines lacking JunD showed increased proliferation and higher cyclin D1 levels. These properties were reminiscent of the effects of oncogenic RAS expression on primary and established cell cultures. Furthermore, JunD -/- fibroblasts exhibited increased p53-dependent apoptosis upon ultraviolet irradiation and were sensitive to the cytotoxic effects of tumor necrosis factor-alpha. The antiapoptotic role of JunD was confirmed using an in vivo model of TNF-mediated hepatitis. The authors proposed that JunD protects cells from senescence, or apoptotic responses to stress stimuli, by acting as a modulator of the signaling pathways that link RAS to p53.Synonyms: JUND, Transcription factor jun-D

Gen-ID: 3727

NCBI Accession: NP_005345

UniProt: P17535