ERK1/2 Antikörper (pThr187, pTyr204)

Produktnummer ABIN197000

Der Kaninchen Polyklonal Anti-ERK1/2-Antikörper wurde für WB, IHC (p) und IF validiert. Er ist geeignet, ERK1/2 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ERK1/2 Antikörper (pThr187, pTyr204) (ABIN197000)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1/2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Produktdetails anti-ERK1/2 Antikörper

Bindungsspezifität: pThr187, pTyr204

Spezifität: This antibody detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at Tyrosine 204.

Aufreinigung: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from Human p44/42 MAP Kinase around the phosphorylation site of Tyrosine 204 (T-E-Yp-V-A).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: 1. TETE HANNKEN, et al. (2000) Am Soc Nephrol 11: 1387-13972. Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 1623. Jingui Yu, et al. (2005) Anesth Analg 101: 315-3214. Hironobu Ihn et al. (2000) Immunology 165: 2149-2155

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Andere Bezeichnung: ERK1 / ERK2

Hintergrund: Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). Both kinases are known to weakly autophosphorylate on tyrosine.Synonyms: ERK-1/ERK-2, Extracellular signal regulated kinase 1/2, Insulin stimulated MAP2 kinase, MAPK1/MAPK2, Microtubule associated protein 2 kinase, Mitogen activated protein kinase 1/2/3, P42/P44-MAPK