GATA1 Antikörper (pSer142)

Produktnummer ABIN196684

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GATA1 in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für GATA1 Antikörper (pSer142) (ABIN196684)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GATA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GATA1 Antikörper

Bindungsspezifität: pSer142

Spezifität: GATA-1 (phospho-Ser142) antibody detects endogenous levels of GATA-1 only when phosphorylated at serine 142.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Synthetic phosphopeptide derived from human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).

Anwendungsinformationen

Applikationshinweise: Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl containing 0.02 % Sodium Azide as preservative and 50 % glycerol as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Andere Bezeichnung: GATA1

Hintergrund: GATA1 (Globin transcription factor 1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. It belongs to the GATA family of transcription factors. GATA1 is a transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. The protein also plays an important role in erythroid development by regulating the switch from fetal hemoglobin production to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. Acquired somatic mutations in GATA1 occur in virtually all children with Down's Syndrome, congenital transient myeloproliferative syndrome (TMD) and acute megakaryocytic leukemia.Synonyms: ERYF1, Eryf1, Erythroid transcription factor, GATA-1, GATA-binding factor 1, GF-1, GF1, NF-E1 DNA-binding protein

Gen-ID: 2623

NCBI Accession: NP_002040

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin