EpCAM Antikörper

Produktnummer ABIN1960146

Produktdetails anti-EpCAM Antikörper

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: IHC-plus™

Spezifität: Recognizes the 34kD cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM). This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumor specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells, and de novo expression being seen in dysplastic squamous epithelium.

Aufreinigung: Purified IgG

Immunogen: HT29 carcinoma cell line.

Type of Immunogen: Cells

Klon: HEA125

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:1), IHC, IHC-Fr (1:20), IHC-P (1:50)

Usage: Flow Cytometry: Use 5 μL of the suggested working dilution to label 10^6 cells or 100 μL cell suspension. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin sections e. g. 0.1 % pronase, 10 minutes. Histology Positive Control Tissue: Human colon & appendix.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.3, 0.09 % sodium azide, 0.5 % BSA.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: ESA / EPCAM (EPCAM Produkte)

Hintergrund: Name/Gene ID: EPCAM

Synonyms: EPCAM, 323/A3, ACSTD1, 17-1A, CD326, EGP, EGP34, Epithelial glycoprotein, GA733-2, HNPCC8, Ep-CAM, ESA, HEGP314, KS 1/4 antigen, KS1/4, KSA, M1S2, MIC18, MK-1, MH99, TROP1, TACST-1, TACSTD1, CD326 antigen, CO-17A, DIAR5, EGP-2, EGP314, EGP40, Epithelial glycoprotein 314, HEA125, Ly74, M4S1

Gen-ID: 4072