ERI1 Antikörper

Produktnummer ABIN1944959

Der Kaninchen Polyklonal Anti-ERI1-Antikörper wurde für WB validiert. Er ist geeignet, ERI1 in Proben von Human, Maus und Ratte zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für ERI1 Antikörper (ABIN1944959)

Target: ERI1 (Exoribonuclease 1 (ERI1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-ERI1 Antikörper

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C,-20 °C

Referenzen für anti-ERI1 Antikörper (ABIN1944959)

Yang, Purdy, Marzluff, Dominski: "Characterization of 3'hExo, a 3' exonuclease specifically interacting with the 3' end of histone mRNA." in: The Journal of biological chemistry, Vol. 281, Issue 41, pp. 30447-54, (2006) (PubMed).

Kennedy, Wang, Ruvkun: "A conserved siRNA-degrading RNase negatively regulates RNA interference in C. elegans." in: Nature, Vol. 427, Issue 6975, pp. 645-9, (2004) (PubMed).

Details zu ERI1

Target: ERI1 (Exoribonuclease 1 (ERI1))

Andere Bezeichnung: ERI1

Hintergrund: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication- dependent histone pre-mRNAs.

Molekulargewicht: 40064 Da

Gen-ID: 90459

UniProt: Q8IV48