Der Kaninchen Polyklonal Anti-PHKB Antikörper wurde für WB validiert. Er ist geeignet, PHKB in Proben von Human und Maus zu detektieren. Es sind 3+ Publikationen verfügbar.
PHKB
Reaktivität: Human
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
AbBy Fluor® 750
Applikationshinweise
WB: 1:500- 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lagerung
4 °C,-20 °C
Oppermann, Gnad, Olsen, Hornberger, Greff, Kéri, Mann, Daub: "Large-scale proteomics analysis of the human kinome." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1751-64, (2009) (PubMed).
Beausoleil, Villén, Gerber, Rush, Gygi: "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." in: Nature biotechnology, Vol. 24, Issue 10, pp. 1285-92, (2006) (PubMed).
Wüllrich-Schmoll, Kilimann: "Structure of the human gene encoding the phosphorylase kinase beta subunit (PHKB)." in: European journal of biochemistry / FEBS, Vol. 238, Issue 2, pp. 374-80, (1996) (PubMed).
Target
PHKB
(Phosphorylase Kinase, beta (PHKB))
Andere Bezeichnung
PHKB
Hintergrund
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.