OSBPL5 Antikörper (N-Term)

Produktnummer ABIN190918

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch OSBPL5 in WB, ELISA, IF, FACS und IHC. Er zeigt eine Reaktivität gegenüber Human und Ratte.

Kurzübersicht für OSBPL5 Antikörper (N-Term) (ABIN190918)

Target: OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser OSBPL5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-OSBPL5 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: ORP5 (OBPH1)

Sequenz: KEEAFLRRRF SLC

Spezifität: This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1, NP_001137535.1) encode the same isoform.

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KEEAFLRRRFSLC, from the N Terminus of the protein sequence according to NP_065947.1, NP_663613.1, NP_001137535.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Kidney and Liver. Recommended concentration: 5 μg/mL.

Western Blot: Approx. 100 kDa band observed in lysates of cell line Jurkat and in Rat Spleen lysates (calculated MW of 98.4 kDa according to Human NP_065947.1 and 101.7 kDa according to Rat NP_001015024.2). Recommended concentration: 0.03=0.3 μg/mL. Primary

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu OSBPL5

Target: OSBPL5 (Oxysterol Binding Protein-Like 5 (OSBPL5))

Andere Bezeichnung: OSBPL5

Hintergrund: OSBPL5, ORP5, OBPH1, FLJ31948, KIAA1534, DKFZp586H1719, oxysterol binding protein-like 5, OSBP-related protein 5, oxysterol-binding protein homologue 1, oxysterol-binding protein-related protein 5, FLJ42929, oxysterol-binding protein-like protein 5

Gen-ID: 114879, 79196

NCBI Accession: NP_065947, NP_663613, NP_001137535