CCKAR Antikörper (Internal Region)

Produktnummer ABIN190898

Der Ziege Polyklonal Anti-CCKAR-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, CCKAR in Proben von Ratte zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für CCKAR Antikörper (Internal Region) (ABIN190898)

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CCKAR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Güteklasse: Verified

Produktdetails anti-CCKAR Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: CCKA Receptor (mouse)

Sequenz: KFDASQKKSA KEKR

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KFDASQKKSAKEKR, from the internal region of the protein sequence according to NP_033957.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx. 75 kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4 kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J. Int J Endocrinol. 2009,2009:875167, PMID: 20011057.

Peptide ELISA: antibody detection limit dilution 1:64000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-CCKAR Antikörper (ABIN190898)

El-Kouhen, Morisset: "Control of somatostatin (SS) secretion by CCK-1 and CCK-2 receptors' occupation in RIN-14B cells, a rat pancreatic islet cell line." in: Pancreas, Vol. 39, Issue 2, pp. 127-34, (2010) (PubMed).

El-Kouhen, Morisset: "Cholecystokinin and Somatostatin Negatively Affect Growth of the Somatostatin-RIN-14B Cells." in: International journal of endocrinology, Vol. 2009, pp. 875167, (2009) (PubMed).

Details zu CCKAR

Target: CCKAR (Cholecystokinin A Receptor (CCKAR))

Andere Bezeichnung: Cckar

Hintergrund: Cckar, cholecystokinin A receptor , AW106902

Gen-ID: 12425, 24889

NCBI Accession: NP_033957

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Feeding Behaviour