Neuroligin 2 Antikörper (Internal Region)

Produktnummer ABIN190848

Dieses Anti-Neuroligin 2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Neuroligin 2 in WB, ELISA, IF und FACS. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Neuroligin 2 Antikörper (Internal Region) (ABIN190848)

Target: Neuroligin 2 (NLGN2)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Neuroligin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Neuroligin 2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Neuroligin 2

Sequenz: NPPDTDIRDP GKK

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NPPDTDIRDPGKK, from the internal region of the protein sequence according to NP_065846.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 90 kDa band observed in Human Pancrease lysates (calculated MW of 90.8 kDa according to NP_065846.1). Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Mouse and

Peptide ELISA: antibody detection limit dilution 1:4000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentrati

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Neuroligin 2 Antikörper (ABIN190848)

Zelano, Berg, Thams, Hailer, Cullheim: "SynCAM1 expression correlates with restoration of central synapses on spinal motoneurons after two different models of peripheral nerve injury." in: The Journal of comparative neurology, Vol. 517, Issue 5, pp. 670-82, (2009) (PubMed).

Details zu Neuroligin 2

Target: Neuroligin 2 (NLGN2)

Andere Bezeichnung: NLGN2

Hintergrund: Neuroligin 2 , KIAA1366, NLGN2

Gen-ID: 57555, 216856, 117096

NCBI Accession: NP_065846

Pathways: Cell-Cell Junction Organization, Synaptic Membrane