ASS1 Antikörper (Internal Region)

Produktnummer ABIN190834

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ASS1 in WB, ELISA, IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für ASS1 Antikörper (Internal Region) (ABIN190834)

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ASS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-ASS1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Argininosuccinate synthetase 1

Sequenz: ENPKNQAPPG LYTKTQD

Spezifität: The variants represent identical protein (NP_000041.2 and NP_446464.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-ENPKNQAPPGLYTKTQD

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48 kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5 kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6 kDa according to

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-ASS1 Antikörper (ABIN190834)

Corbin, Pendleton, Solomonson, Eichler: "Phosphorylation of argininosuccinate synthase by protein kinase A." in: Biochemical and biophysical research communications, Vol. 377, Issue 4, pp. 1042-6, (2008) (PubMed).

Details zu ASS1

Target: ASS1 (Argininosuccinate Synthase 1 (ASS1))

Andere Bezeichnung: ASS1

Hintergrund: Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase

Gen-ID: 445, 11898, 25698

NCBI Accession: NP_000041, NP_446464

Pathways: Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin