TIA1 Antikörper (Internal Region)

Produktnummer ABIN190808

Der Ziege Polyklonal Anti-TIA1-Antikörper wurde für WB, IF, IHC und ELISA validiert. Er ist geeignet, TIA1 in Proben von Human zu detektieren.

Kurzübersicht für TIA1 Antikörper (Internal Region) (ABIN190808)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TIA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-TIA1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TIA1

Sequenz: PKSTYESNTK Q

Spezifität: This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PKSTYESNTKQ, from the internal region of the protein sequence according to NP_071320.1, NP_071505.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Thyroid. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8 kDa according to NP_071320.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band at 80 kDa and a very strong 26 kDa is a

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Andere Bezeichnung: TIA1

Hintergrund: TIA1, TIA1 cytotoxic granule-associated RNA binding protein , TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)

Gen-ID: 7072, 21841, 312510

NCBI Accession: NP_071320, NP_071505