GABPB2 Antikörper (Internal Region)

Produktnummer ABIN190778

Der Ziege Polyklonal Anti-GABPB2-Antikörper wurde für WB, ELISA, IHC, IF und FACS validiert. Er ist geeignet, GABPB2 in Proben von Human zu detektieren.

Kurzübersicht für GABPB2 Antikörper (Internal Region) (ABIN190778)

Target: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GABPB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-GABPB2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: GABPB2

Sequenz: SSENSSKATD ETG

Kreuzreaktivität: Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2, NP_002032.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 45+38 kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5 kDa according to NP_005245.2, NP_001307844.1 and of 38.1 kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GABPB2

Target: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Andere Bezeichnung: GABPB2

Hintergrund: GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53 kDa, GA binding protein transcription factor, beta subuni

Gen-ID: 2553

NCBI Accession: NP_005245, NP_002032, NP_001307844