Glucose-6-Phosphate Dehydrogenase Antikörper (AA 308-320)

Produktnummer ABIN190766

Der Ziege Polyklonal Anti-Glucose-6-Phosphate Dehydrogenase-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, Glucose-6-Phosphate Dehydrogenase in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Glucose-6-Phosphate Dehydrogenase Antikörper (AA 308-320) (ABIN190766)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glucose-6-Phosphate Dehydrogenase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-Glucose-6-Phosphate Dehydrogenase Antikörper

Bindungsspezifität: AA 308-320

Verwendungszweck: G6PD (aa 308 - 320)

Sequenz: STNSDDVRDE KVK

Spezifität: This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 , NP_001035810.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 55-60 kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5 kDa according to NP_000393.4 and 59.3 kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Glucose-6-Phosphate Dehydrogenase Antikörper (ABIN190766)

Hwang, Mruk, Rahighi, Raub, Chen, Dorn, Horikoshi, Wakatsuki, Chen, Mochly-Rosen: "Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator." in: Nature communications, Vol. 9, Issue 1, pp. 4045, (2019) (PubMed).

Details zu Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Andere Bezeichnung: G6PD

Hintergrund: G6PD, glucose-6-phosphate dehydrogenase, G6PD1, glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, G6PD

Gen-ID: 2539, 14381, 24377

NCBI Accession: NP_000393, NP_001035810

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones