FH Antikörper (Internal Region)

Produktnummer ABIN190758

Dieser Anti-FH-Antikörper ist ein Ziege-Polyklonal-Antikörper zum Nachweis von FH in WB, ELISA, IHC und EIA. Geeignet für Human.

Kurzübersicht für FH Antikörper (Internal Region) (ABIN190758)

Target: FH (Fumarate Hydratase (FH))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Güteklasse: Verified

Produktdetails anti-FH Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Fumarase / FH

Sequenz: HPNDHVNKSQ SSND

Homologie: Human, Mouse, Rat, Dog

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

DS WB Results: Approx 48 kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.

DS IHC Results: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu FH

Target: FH (Fumarate Hydratase (FH))

Andere Bezeichnung: Fumarate Hydratase

Hintergrund: Fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata

Gen-ID: 2271

NCBI Accession: NP_000134