PsbP Antikörper

Produktnummer ABIN190744

Produktdetails

Target: PsbP

Reaktivität: Arabidopsis thaliana, Gerste, Spinach

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Kreuzreaktivität (Details): No cross-reactivity with: Synechococcus

Homologie: dicots including: Pisum sativum, Solanum lycopersicum, monocots including: Oryza sativa, trees including: Populus balsamifera, Pinus monticola

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 28 / 23 kDa

Aufreinigung: affinity purified

Immunogen: native, purified 23 kDa protein from Spinacia oleracea

Anwendungsinformationen

Applikationshinweise: Recommended Dilution 1 : 2000 - 1 : 5000 with standard ECL (WB).

Kommentare:

Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS pH 7.4

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Wang, Gu, Colby, Shindo, Balamurugan, Waldmann, Kaiser, van der Hoorn: "Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana." in: Nature chemical biology, Vol. 4, Issue 9, pp. 557-63, (2008) (PubMed).

Antigendetails

Target: PsbP

Hintergrund: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33-kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Molekulargewicht: expected: 28 kDa, apparent: 23 kDa