PsbP Antikörper

Produktnummer ABIN190744

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PsbP in WB. Es zeigt Reaktivität gegenüber Proben von Arabidopsis thaliana, Gerste, Chlamydomonas reinhardtii, Pinus banksiana, Spinach und Triticum aestivum. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für PsbP Antikörper (ABIN190744)

Target: PsbP

Reaktivität: Arabidopsis thaliana, Gerste, Chlamydomonas reinhardtii, Pinus banksiana, Spinach, Triticum aestivum

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

Kreuzreaktivität (Details): Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Not reactive in: Synechococcus sp. PCC 7942

Homologie: Musa acuminata,Oryza sativa,Pinus monticola,Pisum sativum,Populus balsamifera,Solanum lycopersicum

Aufreinigung: Protein G purified

Immunogen: native

Anwendungsinformationen

Applikationshinweise: 1 : 2000-1 : 5000 (WB)

Kommentare:

Load per well on cell extract of Pinus banksiana (Jack Pine) was 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, (2011) (PubMed).

Wang, Gu, Colby, Shindo, Balamurugan, Waldmann, Kaiser, van der Hoorn: "Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana." in: Nature chemical biology, Vol. 4, Issue 9, pp. 557-63, (2008) (PubMed).

Antigendetails

Target: PsbP

Hintergrund: PSII reaction centre components are generating the redox potential required to drive highly oxidizing water splitting reaction. Four Mn atoms are present on a lumenal surface and form the catalyctic site of the water-splitting reaction which is in close association with the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) extrinistic subunits of oxygen evolving complex OEC. A 33- kDa extrinsic protein is also termed the Mn-stabilizing protein (MSP), however recent evidences shown that it is C-terminal domain of PsbA (D1) protein which is involved in in the assembly and stabilization of the OEC.

Molekulargewicht: 28 | 23 kDa

UniProt: P12302