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Nitrogenase Antikörper

This anti-Nitrogenase antibody (ABIN190742) is a Chicken Polyclonal antibody detecting Nitrogenase in WB. Suitable for Rhodopseudomonas palustris.
Produktnummer ABIN190742

Kurzübersicht für Nitrogenase Antikörper (ABIN190742)

Target

Nitrogenase (Nifh)

Reaktivität

Rhodopseudomonas palustris

Wirt

Huhn

Klonalität

Polyklonal

Applikation

Western Blotting (WB)
  • Kreuzreaktivität (Details)

    No cross-reactivity with: Synechococcus sp. PCC 7942 and Synechocystis

    Homologie

    Desulfotomaculum reducens (strain MI-1),Clostridium cellobioparum, Frankia sp. (strain EAN1pec), Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp , Rhodopseudomonas palustris TIE-1 strain, alpha,gamma,beta proteobacteria, enterobacteria, low GC gram+, high GC gram +, euryachaeotes, Azotobacter vinelandii (Gram-), Klebsiella pneumonia, cyanobacteria, Enterobacter genera, able to fix atmoshperic nitrogen

    Produktmerkmale

    Expected / apparent Molecular Weight of the Antigene: 27 / 32.5 kDa

    Aufreinigung

    affinity purified

    Immunogen

    KLH-conjugated synthetic peptide derived from known bacterial NifH subunits of bacterial nitrogenase enzymes of the FeMoCo type including Synechoccocus sp. Q2JP78 , Trichodesmium theibautii, Anabaena sp. P33178 and Nostoc sp. Q51296
  • Applikationshinweise

    Recommended Dilution 1 : 2 000 (WB).

    Kommentare

    This antibody is not suitable for immunolocalization studies on bacterial cultures.An enzyme involved in chlorophyll synthesis, present in all cyanobacteria (fixing and non-nitrogen fixing) is a member of the NifH family/superfamily. Agrisera anti-NifH antibody will not show a strong reactivity to this target.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1.36 µg/µL

    Buffer

    PBS pH 8.0+ 0.02 % sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
    Make aliquots to avoid repreated freeze-thaw cycles and working with a stock.

    Lagerung

    4 °C
  • Halimatul, Ehira, Awai: "Fatty alcohols can complement functions of heterocyst specific glycolipids in Anabaena sp. PCC 7120." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 178-83, (2014) (PubMed).

    Levitan, Brown, Sudhaus, Campbell, LaRoche, Berman-Frank: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." in: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).

  • Target

    Nitrogenase (Nifh)

    Hintergrund

    Nitrogenase is involved in biological fixation of atmospheric nitrogen to ammonia. Alternative protein names: nitrogenase component II, nitrogenase Fe protein, nitrogenase reductase, FeMoCo-nitrogenase.

    Molekulargewicht

    expected: 27 kDa, apparent: 32.5 kDa

    UniProt

    P26254, Q2JP78, P33178, Q51296
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