Superoxide dismutase copper chaperone Antikörper (cleaved)

Produktnummer ABIN190735

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Superoxide dismutase copper chaperone in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana, Mustard, Oryza sativa und Mais (Zea mays).

Kurzübersicht für Superoxide dismutase copper chaperone Antikörper (cleaved) (ABIN190735)

Target: Superoxide dismutase copper chaperone (CCS) (Copper Chaperone For Superoxide Dismutase (CCS))

Reaktivität: Arabidopsis thaliana, Mustard, Oryza sativa, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Superoxide dismutase copper chaperone Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Superoxide dismutase copper chaperone Antikörper

Bindungsspezifität: cleaved

Kreuzreaktivität (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: dicots including: Glycine max, Solanum tuberosum

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 33.8 / 37 kDa (Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: The antibody was raised to the Arabidopsis thaliana full length CCs protein Q4ZJI5, fused to His-tag. Overexpressed using pET28a vector in Bl21 E. coli (Cd+) and purified using His-column. After purification, the His tag was cleaved using thrombin and then theprotein was purified using Resource-Q column connected to HPLC.

Anwendungsinformationen

Applikationshinweise: 1 : 1000 with alkaline phosphatase (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Details zu Superoxide dismutase copper chaperone

Target: Superoxide dismutase copper chaperone (CCS) (Copper Chaperone For Superoxide Dismutase (CCS))

Andere Bezeichnung: CCS

Hintergrund: AGI Code: At1g12520
Copper is an essential element for function of chloroplasts. It is a co-factor for superoxide dismutase (SOD) and for plastocyanin. Copper is transported to chloroplasts with a help of Copper Chaperones, which carry out the delivery of copper from transporters to targets. In the last step chaperone binds to a copper dependent enzyme and inserts the copper ions into its active site. The Arabidopsis thaliana gene AtCCS encodes a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD.

Molekulargewicht: expected: 33.8 kDa, apparent: 37 kDa (Arabidopsis thaliana)

UniProt: Q4ZJI5

Pathways: Transition Metal Ion Homeostasis