Rubisco Antikörper

Produktnummer ABIN190723

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Rubisco in WB und IL. Es zeigt Reaktivität gegenüber Proben von Arabidopsis thaliana, Aucuba japonica, Gerste, Blue-Green Algae (Fremyella), Manihot esculenta, Oryza sativa, Pea, Physcomytrium Patens, Poplar (Populus), Potato, Salsola laricifolia, Soybean, Spinach, Synechococcus sp., Synechocystis, Mais (Zea mays) und Anabaena variabilis. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für Rubisco Antikörper (ABIN190723)

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reaktivität: Arabidopsis thaliana, Aucuba japonica, Gerste, Blue-Green Algae (Fremyella), Manihot esculenta, Oryza sativa, Pea, Physcomytrium Patens, Poplar (Populus), Potato, Salsola laricifolia, Soybean, Spinach, Synechococcus sp., Synechocystis, Mais (Zea mays), Anabaena variabilis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Rubisco Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-Rubisco Antikörper

Verwendungszweck: Anti-Rubisco | 557 kDa hexadecamer

Kreuzreaktivität (Details): Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Not reactive in: Chlamydomonas reinhardtii (immulolocalization)

Homologie: higher plants,algae,Anthoceros agrestis,Nannochloropsis sp.

Aufreinigung: Serum

Immunogen: purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

Anwendungsinformationen

Applikationshinweise: 1 : 10 000-1 : 20 000 on 0,5-10 μg total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)

Kommentare:

RbcS subunit is not detected by this antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen für anti-Rubisco Antikörper (ABIN190723)

Kissen, Hyldbakk, Wang, Sørmo, Rossiter, Bones: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).

Li, Zhu, Zeng, Zhang, Ye, Ou, Rehman, Heuer, Chen: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." in: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).

Details zu Rubisco

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Andere Bezeichnung: Rubisco

Hintergrund: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Molekulargewicht: 53-55 | 53-55 kDa

Gen-ID: 2715621

UniProt: P00875, Q43832