Rubisco Antikörper

Produktnummer ABIN190723

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Rubisco in WB. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana, Spinach, Chlamydomonas, Potato, Soybean und Synechocystis PCC 6803 und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für Rubisco Antikörper (ABIN190723)

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Reaktivität: Arabidopsis thaliana, Spinach, Chlamydomonas, Potato, Soybean, Synechocystis PCC 6803

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Rubisco Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Rubisco Antikörper

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 53-55 / 53-55 kDa

Aufreinigung: serum

Immunogen: purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000)

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: 1 : 10 000 - 1 : 20 000 on 0.5-10 ug total cellular protein/lane and standard ECL(WB).

Kommentare:

RbcS subunit is not detected by this antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 µL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen für anti-Rubisco Antikörper (ABIN190723)

Kissen, Hyldbakk, Wang, Sørmo, Rossiter, Bones: "Ecotype dependent expression and alternative splicing of epithiospecifier protein (ESP) in Arabidopsis thaliana." in: Plant molecular biology, Vol. 78, Issue 4-5, pp. 361-75, (2012) (PubMed).

Li, Zhu, Zeng, Zhang, Ye, Ou, Rehman, Heuer, Chen: "Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots." in: Proteome science, Vol. 8, pp. 10, (2010) (PubMed).

Details zu Rubisco

Target: Rubisco (RuBisCO) (Ribulose-1,5-Bisphosphate Carboxylase/oxygenase (RuBisCO))

Andere Bezeichnung: Rubisco

Hintergrund: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

Molekulargewicht: expected: 53-55 kDa, apparent: 53-55 kDa

UniProt: Q43832, P00875