GLU I (PR-2) Antikörper

Produktnummer ABIN190719

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-GLU I (PR-2) Antikörper (ABIN190719) detektiert spezifisch GLU I (PR-2) in IL und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana tabacum, Phalaenopsis equestris, Poplar (Populus), Potato, Tomato und Vitis vinifera.

Kurzübersicht für GLU I (PR-2) Antikörper (ABIN190719)

Target: GLU I (PR-2)

Reaktivität: Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana tabacum, Phalaenopsis equestris, Poplar (Populus), Potato, Tomato, Vitis vinifera

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunolocalization (IL), Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-PR-2 | GLU I | Class I beta-1,3-glucanase

Kreuzreaktivität (Details): Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in: Arabidopsis thaliana

Homologie: Dicots,Oryza sativa,Prunus persica

Immunogen: Purified tobacco class I

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: 8 μg/mL (WB)

Kommentare:

Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water

Buffer: Total IgG in PBS pH 7.4. (without Ca++).

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen

Beffa, Neuhaus, Meins: "Physiological compensation in antisense transformants: specific induction of an \" glucan endo-1,3-beta-glucosidase in plants infected with necrotizing viruses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 19, pp. 8792-6, (1993) (PubMed).

Antigendetails

Target: GLU I (PR-2)

Hintergrund:

Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase

Additional information: For more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51.This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

Molekulargewicht: 37 | 33 kDa

Gen-ID: 107801151

UniProt: P27666