This antibody detects lead in the ppb range. Indirect ELISA endpoint titer is >1: 100,000.
Aufreinigung
Whole Antiserum
Immunogen
Lead conjugated to keyhole limpet hemocyanin (KLH) by proprietary chemistry.
Isotyp
IgM
Applikationshinweise
Indirect ELISA: optimized for lead-RSA (rabbit serum albumin) on solid phase at 200 ng/well. Ascites diluted 1:100,000 produces an O.D. signal of 0.5. Competition ELISA: with lead-RSA on the solid phase at 200 ng/well, 50 % inhibition of antibody binding is achieved with Pb2+ at 800 ng/well. These are recommended concentrations. End users should determine optimal concentrations for their applications.Not all applications have been investigated.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Dilute in PBS or medium that is identical to that used in the assay system.
Konzentration
Lot specific
Buffer
Whole antiserum
Lagerung
-20 °C
Informationen zur Lagerung
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Target
Lead
Substanzklasse
Chemical
Hintergrund
Exposure to potentially neurotoxic levels of lead occurs in approximately 9 % of American children under 6 years of age, and many urban dwellings still contain dangerous levels of lead paint.