CLP1 Antikörper (AA 142-341)

Produktnummer ABIN1886042

Der Kaninchen Polyklonal anti-CLP1 Antikörper wird verwendet zum Nachweis von CLP1 in Proben von Human. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für CLP1 Antikörper (AA 142-341) (ABIN1886042)

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CLP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CLP1 Antikörper

Bindungsspezifität: AA 142-341

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 142 and 341 of HEAB

Anwendungsinformationen

Applikationshinweise: Suggested dilutions:
Immunohistochemistry: 1.100-1.250
Immunofluorescence: 1.100-1.200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 20 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: Biohazard Informations: This product contains thimerosal which is hazardous.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu CLP1

Target: CLP1 (Cleavage and Polyadenylation Factor I Subunit 1 (CLP1))

Andere Bezeichnung: HEAB

Hintergrund: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids.dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component.Appears to have roles in both tRNA splicing and mRNA 3'-end formation.Component of the tRNA splicing endonuclease complex.Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing, this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA.Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation.Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC).However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Molekulargewicht: 48 kDa

Gen-ID: 10978

Pathways: Ribonucleoprotein Complex Subunit Organization