MBNL1 Antikörper (C-Term)

Produktnummer ABIN1881533

Der Kaninchen Polyklonal Anti-MBNL1-Antikörper wurde für WB validiert. Er ist geeignet, MBNL1 in Proben von Human zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für MBNL1 Antikörper (C-Term) (ABIN1881533)

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBNL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB42214

Produktdetails anti-MBNL1 Antikörper

Bindungsspezifität: AA 255-284, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MBNL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from the C-terminal region of human MBNL1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-MBNL1 Antikörper (ABIN1881533)

Sen, Talukdar, Liu, Tam, Reddy, Webster: "Muscleblind-like 1 (Mbnl1) promotes insulin receptor exon 11 inclusion via binding to a downstream evolutionarily conserved intronic enhancer." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25426-37, (2010) (PubMed).

Arambula, Ramisetty, Baranger, Zimmerman: "A simple ligand that selectively targets CUG trinucleotide repeats and inhibits MBNL protein binding." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 38, pp. 16068-73, (2009) (PubMed).

Cardani, Baldassa, Botta, Rinaldi, Novelli, Mancinelli, Meola: "Ribonuclear inclusions and MBNL1 nuclear sequestration do not affect myoblast differentiation but alter gene splicing in myotonic dystrophy type 2." in: Neuromuscular disorders : NMD, Vol. 19, Issue 5, pp. 335-43, (2009) (PubMed).

Holt, Jacquemin, Fardaei, Sewry, Butler-Browne, Furling, Brook, Morris: "Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle." in: The American journal of pathology, Vol. 174, Issue 1, pp. 216-27, (2009) (PubMed).

Teplova, Patel: "Structural insights into RNA recognition by the alternative-splicing regulator muscleblind-like MBNL1." in: Nature structural & molecular biology, Vol. 15, Issue 12, pp. 1343-51, (2008) (PubMed).

Details zu MBNL1

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Andere Bezeichnung: MBNL1

Hintergrund: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Molekulargewicht: 41817

NCBI Accession: NP_066368, NP_997175, NP_997176, NP_997177, NP_997178, NP_997179, NP_997180

UniProt: Q9NR56

Pathways: Ribonucleoprotein Complex Subunit Organization