pan Keratin Antikörper

Produktnummer ABIN187832

Der Kaninchen Polyklonal Anti-pan Keratin-Antikörper wurde für IHC (p) und IHC (fro) validiert. Er ist geeignet, pan Keratin in Proben von Human, Ratte, Maus, Schwein, Hund, Kaninchen, Hamster und Avian zu detektieren.

Kurzübersicht für pan Keratin Antikörper (ABIN187832)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Schwein, Hund, Kaninchen, Hamster, Avian

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser pan Keratin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-pan Keratin Antikörper

Spezifität: Recognizes keratin Species Reactivity: Most mammals and birds.

Immunogen: Keratin extracted from human skin. Antigen Location: Cytoplasm

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry - Frozen sections: Fixation for 3 min. with 1% formaldehyde is recommended. Formaldehyde-fixed, paraffin-embedded tissue sections: Proteolytic pretreatment with pronase or trypsine is recommended. Use at a 1:50 dilution in PBS or Tris. Recommended positive control: Squamous cell carcinoma, skin, cervix. The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.25 mL affinity chromatography purified liquid antiserum.

Lagerung: 4 °C

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Keratin

Hintergrund: This polyclonal antibody reacts with keratin of most mammals and birds. A positive reaction is obtained with all stratified squamous epithelia and in epidermal appendages such as hairfollicles and sebaceous glands. Also the epithelia of the urinary tract, stomach, small and large intestine, uterine endometrium, bile ducts, prostate, kidney collecting tubules, thymic reticulum cells, mesothelium and to a lesser extent hepatocytes, adenocarcinomas, transitional cell carcinomas and anaplastic carcinomas can be identified by this antiserum. Renal cell adenocarcinomas and small cell anaplastic lung tumors may remain negative when examined in paraffin sections. Hepatocytes and pancreatic acini show a positive staining. Nearly all cultured epithelial cells are positive, all non-epithelial cells are negative.