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SUN5 Antikörper

SUN5 Reaktivität: Human, Maus, Ratte WB Wirt: Kaninchen Polyclonal unconjugated
Independent Validation (1 Validierung)
Produktnummer ABIN1876373
$355.71
Zzgl. Versandkosten $45.00
100 μL
Lieferung in 11 bis 13 Werktagen
  • Target Alle SUN5 Antikörper anzeigen
    SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))
    Reaktivität
    Human, Maus, Ratte
    Wirt
    • 26
    • 2
    Kaninchen
    Klonalität
    • 28
    Polyklonal
    Konjugat
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser SUN5 Antikörper ist unkonjugiert
    Applikation
    • 13
    • 13
    • 8
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB)
    Produktmerkmale
    Expected molecular weight of target: 379 kDa
    Aufreinigung
    Affinity purification
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:500-1:2000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #102344 (Immunocytochemistry)
    'Independent Validation' Siegel
    by
    Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Developmental Biology, Georg-August-University Göttingen
    No.
    #102344
    Datum
    19.12.2017
    Antigen
    SUN5
    Chargennummer
    Validierte Anwendung
    Immunocytochemistry
    Positivkontrolle
    NIH/3T3 mouse embryonic fibroblast cells overexpressing HA- and myc-tagged SPAG4L-2/SUN5
    Negativkontrolle
    NIH/3T3 mouse embryonic fibroblast cells overexpressing HA- and myc-tagged SPAG4
    Bewertung

    Passed. ABIN1876373 is specific for SPAG4L-2/SUN5 and did not detect SPAG4 when ectopically expressed. Unspecific binding to other cellular proteins is nearly absent.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN1876373
    Sekundärantikörper
    goat anti-rabbit IgG DyLight488 (Thermo Scientific, 35553, lot KA130088)
    Full Protocol
    • NIH/3T3 cells (ATCC, CRL-1658) are grown in DMEM+GlutaMAX (Gibco, 31966-021, Lot 1852045) supplemented with fetal bovine serum (Gibco 270-106) and Pen/Strep (Gibco 15140), at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with HA/myc-tagged SPAG4 expression plasmid or HA/myc-tagged SPAG4L-2/SUN5 (Frohnert et al., 2011) expression plasmid using EndofectinMax (GeneCopoeia) following the manufacturer's instructions.
    • Grow cells for 24h.
    • Fix cells in 3.7% paraformaldehyde (in PBS) for 15min at 4°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at room temperature.
    • Incubate cells with primary
      • rabbit anti-SUN5 antibody (antibodies-online, ABIN1876373) diluted 1:100 in PBS ON at 4° and
      • mouse anti-myc tag antibody clone (9E10) diluted 1:100 in PBS ON at 4°C.
    • Wash cells with TBST (50mM Tris-HCl, pH 7.4, 150mM NaCl, 0.1% Tween 20) for 15min at RT.
    • Incubate cells with secondary
      • goat anti-rabbit IgG DyLight488 (Thermo Scientific, 35553, lot KA130088) diluted 1:1000 in PBS for 1h at 37°C and
      • goat anti-mouse IgG (H+L) Alexa Fluor 555 (Invitrogen, A21422, lot 948498) diluted 1:1000 in PBS for 1h at 37°C.
    • Counterstain DNA with DAPI (4,6-Diamidino-2-phenylindole; Sigma D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Anmerkungen
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C.
  • Target
    SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))
    Andere Bezeichnung
    SPAG4L (SUN5 Produkte)
    Synonyme
    SPAG4L, TSARG4, dJ726C3.1, 1700021O15Rik, SPAG4L-2, SRG4, Spag4l, Sad1 and UNC84 domain containing 5, SUN5, Sun5
    Gen-ID
    140732
    UniProt
    Q8TC36
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