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SUN5 Antikörper

Der Kaninchen Polyklonal Anti-SUN5-Antikörper wurde für WB validiert und wurde unabhängig validiert. Er ist geeignet, SUN5 in Proben von Human, Maus und Ratte zu detektieren.
Produktnummer ABIN1876373

Kurzübersicht für SUN5 Antikörper (ABIN1876373)

Target

Alle SUN5 Antikörper anzeigen
SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))

Reaktivität

Human, Maus, Ratte

Wirt

  • 26
  • 2
Kaninchen

Klonalität

  • 28
Polyklonal

Konjugat

  • 7
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser SUN5 Antikörper ist unkonjugiert

Applikation

  • 20
  • 13
  • 13
  • 4
  • 3
  • 3
  • 1
Western Blotting (WB)
  • Produktmerkmale

    Expected molecular weight of target: 379 kDa

    Aufreinigung

    Affinity purification

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:500-1:2000

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #102344 (Immunocytochemistry)
    'Independent Validation' Siegel
    by
    Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Department of Developmental Biology, Georg-August-University Göttingen
    No.
    #102344
    Datum
    19.12.2017
    Antigen
    SUN5
    Chargennummer
    Validierte Anwendung
    Immunocytochemistry
    Positivkontrolle
    NIH/3T3 mouse embryonic fibroblast cells overexpressing HA- and myc-tagged SPAG4L-2/SUN5
    Negativkontrolle
    NIH/3T3 mouse embryonic fibroblast cells overexpressing HA- and myc-tagged SPAG4
    Bewertung

    Passed. ABIN1876373 is specific for SPAG4L-2/SUN5 and did not detect SPAG4 when ectopically expressed. Unspecific binding to other cellular proteins is nearly absent.

    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN1876373
    Sekundärantikörper
    goat anti-rabbit IgG DyLight488 (Thermo Scientific, 35553, lot KA130088)
    Full Protocol
    • NIH/3T3 cells (ATCC, CRL-1658) are grown in DMEM+GlutaMAX (Gibco, 31966-021, Lot 1852045) supplemented with fetal bovine serum (Gibco 270-106) and Pen/Strep (Gibco 15140), at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with HA/myc-tagged SPAG4 expression plasmid or HA/myc-tagged SPAG4L-2/SUN5 (Frohnert et al., 2011) expression plasmid using EndofectinMax (GeneCopoeia) following the manufacturer's instructions.
    • Grow cells for 24h.
    • Fix cells in 3.7% paraformaldehyde (in PBS) for 15min at 4°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at room temperature.
    • Incubate cells with primary
      • rabbit anti-SUN5 antibody (antibodies-online, ABIN1876373) diluted 1:100 in PBS ON at 4° and
      • mouse anti-myc tag antibody clone (9E10) diluted 1:100 in PBS ON at 4°C.
    • Wash cells with TBST (50mM Tris-HCl, pH 7.4, 150mM NaCl, 0.1% Tween 20) for 15min at RT.
    • Incubate cells with secondary
      • goat anti-rabbit IgG DyLight488 (Thermo Scientific, 35553, lot KA130088) diluted 1:1000 in PBS for 1h at 37°C and
      • goat anti-mouse IgG (H+L) Alexa Fluor 555 (Invitrogen, A21422, lot 948498) diluted 1:1000 in PBS for 1h at 37°C.
    • Counterstain DNA with DAPI (4,6-Diamidino-2-phenylindole; Sigma D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Anmerkungen
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freeze/thaw cycles.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store at -20°C.
  • Target

    SUN5 (Sad1 and UNC84 Domain Containing 5 (SUN5))

    Andere Bezeichnung

    SPAG4L

    Gen-ID

    140732

    UniProt

    Q8TC36
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