HSP70 Antikörper

Produktnummer ABIN187606

Dieses Maus Monoklonal-Antikörper erkennt spezifisch HSP70 in WB, IP, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh) und Affe.

Kurzübersicht für HSP70 Antikörper (ABIN187606)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reaktivität: Human, Rind (Kuh), Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP70 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: W27

Produktdetails anti-HSP70 Antikörper

Spezifität: This clone recognizes both the constitutive (Hsp73) and inducible (Hsp72) forms of Hsp70. Species Reactivity: Reacts with Human, Monkey and Bovine. Other species not tested. Positive Control: BT474 or HeLa cells. Breast carcinoma.

Immunogen: Hsp70 protein from HeLa cells.Produced by fusion between BALB/c spleno-cytes and mouse myeloma NS-1 cells.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry Immunofluorescence (Not assessed) Immunoprecipitation: Use at 2 µg/mg protein lysate. Use Protein A. Western blotting: 0.5-1 µg/ml for 2 hrs. at RT Immunohistology: Formalin/paraffin - Use at 2-4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Clone W27 is a monoclonal antibody against Heat Shock Factor 2. It is available at a concentration of 100ug/500ul in 10 mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide. Purified from ascites fluid by Protein A chromatography.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Andere Bezeichnung: Heat shock protein 70 / HSP70

Hintergrund: A wide variety of environmental and patho-physiological stress conditions trigger the synthesis of a family of proteins known as heat shock proteins, more appropriately called stress response proteins. Many heat shock proteins, including members of the Ssp70 family, are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation and secretion. Hsp70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides and other unknown proteins. Also, Hsp70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs and other damaging conditions.