PARP4 Antikörper

Produktnummer ABIN187331

Produktdetails anti-PARP4 Antikörper

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PARP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This clone (P193-6) reacts with an internal epitope (amino acids 593-599, FSKVEDY) or the minor vault protein (VPARP), which is over-expressed in various human non-P-glycoprotein MDR tumor lines, accordingly to an increase in the number of vault particles.

Immunogen: Clone p193-6 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.

Klon: P193-6

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blotting: (preferabley with ECL) Use and a dilution of 1:10 to 20. Immunohistochemistry: Use at a dilution of 1:10-20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. The following pretreatment is recommended: 10 min with 20 mM glycine in PBS (pH 7.5) and 10 min. with 6 N guanidine hydrochloride in 50 mM Tris-HCL (pH 7.5)1. Optimal conditions for paraffin-embedded tissues have yet to be defined. Flow Cytometry: Optimal conditions have yet to be defined. The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: This antibody contains approximately 250ug of immunoglobulin per ml. The solution contains 1% BSA and 0.1% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu PARP4

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Andere Bezeichnung: PARP4 (PARP4 Produkte)

Synonyme: ADPRTL1 antikoerper, ARTD4 antikoerper, PARP-4 antikoerper, PARPL antikoerper, PH5P antikoerper, VAULT3 antikoerper, VPARP antikoerper, VWA5C antikoerper, p193 antikoerper, Adprtl1 antikoerper, C030027K23Rik antikoerper, E230037B21Rik antikoerper, Gm743 antikoerper, poly(ADP-ribose) polymerase family member 4 antikoerper, poly (ADP-ribose) polymerase family, member 4 antikoerper, PARP4 antikoerper, Parp4 antikoerper

Hintergrund: VPARP is an antibody to the minor vault protein. It reacts with internal epitope Amino Acids 593-599, FSKVEDY, of the minor vault protein.