PARP4 Antikörper (AA 491-494)

Produktnummer ABIN187330

Dieser Maus Monoklonal Antikörper detektiert spezifisch PARP4 in WB, IHC (fro) und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PARP4 Antikörper (AA 491-494) (ABIN187330)

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PARP4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Klon: P193-4

Produktdetails anti-PARP4 Antikörper

Bindungsspezifität: AA 491-494

Spezifität: This clone (P193-4) reacts with an internal epitope (amino acids 491-494, HPGE) or the minor vault protein (VPARP), which is over-expressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.

Immunogen: Clone p193-4 was raised against an E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blotting: (preferabley with ECL) Use and a dilution of 1:10 to 20. Immunohistochemistry: Use at a dilution of 1:10-20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. The following pretreatment is recommended: 10 min with 20 mM glycine in PBS (pH 7.5) and 10 min. with 6 N guanidine hydrochloride in 50 mM Tris-HCL (pH 7.5)1. Optimal conditions for paraffin-embedded tissues have yet to be defined. Flow Cytometry: Optimal conditions have yet to be defined. The optimal dilution for a specific application should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: This antibody contains approximately 250ug of immunoglobulin per ml. The solution contains 1% BSA and 0.1% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu PARP4

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Andere Bezeichnung: PARP4

Hintergrund: VPARP is an antibody to the minor vault protein.