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Vitamin D2 Antikörper (Biotin)

Der Kaninchen Polyklonal Anti-Vitamin D2-Antikörper wurde für ICC, IHC und WB validiert. Er ist geeignet, Vitamin D2 in Proben von Diverse Spezies zu detektieren.
Produktnummer ABIN1872536

Kurzübersicht für Vitamin D2 Antikörper (Biotin) (ABIN1872536)

Target

Vitamin D2

Reaktivität

  • 2
  • 1
Diverse Spezies

Wirt

  • 3
Kaninchen

Klonalität

  • 3
Polyklonal

Konjugat

  • 2
  • 1
Dieser Vitamin D2 Antikörper ist konjugiert mit Biotin

Applikation

  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
  • Verwendungszweck

    Biotin-Linked Polyclonal Antibody to Vitamin D2 (VD2)

    Spezifität

    The antibody is a rabbit polyclonal antibody raised against VD2. It has been selected for its ability to recognize VD2 in immunohistochemical staining and western blotting.

    Aufreinigung

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogen

    The antibody is a rabbit polyclonal antibody raised against VD2 conjugated to biotin.

    Isotyp

    IgG
  • Applikationshinweise

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Kommentare

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Rekonstitution

    Reconstitute in sterile PBS, pH 7.2 - pH 7.4.

    Konzentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handhabung

    Avoid repeated freeze/thaw cycles

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    Vitamin D2

    Hintergrund

    Ergocalciferol, Deltalin, Drisdol, Calcidol
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