SECISBP2 Antikörper (Internal Region)

Produktnummer ABIN185714

Der Ziege Polyklonal Anti-SECISBP2-Antikörper wurde für WB, ELISA, IF, IHC, IP und ICC validiert. Er ist geeignet, SECISBP2 in Proben von Human und Maus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für SECISBP2 Antikörper (Internal Region) (ABIN185714)

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SECISBP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Güteklasse: Verified

Produktdetails anti-SECISBP2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: SBP2

Sequenz: KERQERKQRL QEN

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-KERQERKQRLQEN

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3 μg/mL.

Western Blot: Approx 120 kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 J

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunocytochemistry/Immunofluorescence: This antibody has been successfully used in ICC/IF for C33A cells as described in the following paper: Papp et al, Nucleic Acids Res. 2008 Dec,36(22):7192-206, PMID: 19004874.
Immunoprec

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-SECISBP2 Antikörper (ABIN185714)

Raman, Pitts, Seyedali, Hashimoto, Bellinger, Berry: "Selenoprotein W expression and regulation in mouse brain and neurons." in: Brain and behavior, Vol. 3, Issue 5, pp. 562-74, (2014) (PubMed).

Papp, Wang, Kennedy, Boucher, Zhang, Gladyshev, Singh, Khanna: "Functional characterization of alternatively spliced human SECISBP2 transcript variants." in: Nucleic acids research, Vol. 36, Issue 22, pp. 7192-206, (2008) (PubMed).

Details zu SECISBP2

Target: SECISBP2 (SECIS Binding Protein 2 (SECISBP2))

Andere Bezeichnung: SECISBP2

Hintergrund: SBP2 , RP11-89K14.1, SECISBP2, SECIS binding protein 2 , DKFZp686C09169, OTTHUMP00000064929, OTTHUMP00000064930, OTTHUMP00000064931, OTTHUMP00000064932, selenocysteine insertion sequence binding protein 2

Gen-ID: 79048

NCBI Accession: NP_076982