DSCAM Antikörper (Internal Region)

Produktnummer ABIN185687

Der Ziege Polyklonal anti-DSCAM Antikörper wird verwendet zum Nachweis von DSCAM in Proben von Human. Er wurde validiert für ELISA, WB, FACS und IF.

Kurzübersicht für DSCAM Antikörper (Internal Region) (ABIN185687)

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser DSCAM Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-DSCAM Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: DSCAM

Sequenz: DSWDSAQRTK DVSPQ

Spezifität: This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1).

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DSWDSAQRTKDVSPQ, from the internal region of the protein sequence according to NP_001380.2, NP_996770.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 200-220 kDa band observed in HepG2 cell lysates (calculated MW of 174 kDa according to NP_996770.1 ). This molecular weight is routinely observed by other sources. Recommended concentration: 2-3 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu DSCAM

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Andere Bezeichnung: DSCAM

Hintergrund: DSCAM, Down syndrome cell adhesion molecule , CHD2-42, CHD2-52 , human CHD2-52 down syndrome cell adhesion molecule

Gen-ID: 1826, 13508, 171119

NCBI Accession: NP_001380, NP_001258463

Pathways: Regulation of Cell Size