AIRE Antikörper (Internal Region)

Produktnummer ABIN185643

Dieses Anti-AIRE-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von AIRE in WB, ELISA und IHC. Geeignet für Human und Schwein. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.

Kurzübersicht für AIRE Antikörper (Internal Region) (ABIN185643)

Target: AIRE (Autoimmune Regulator (AIRE))

Reaktivität: Human, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser AIRE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-AIRE Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: AIRE

Sequenz: KDVDLSQPRK GRKP

Kreuzreaktivität: Rind (Kuh), Hund, Human, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Thymus shows nuclear staining in select thymocytes. This antibody has been successfully used in IHC on Human: Ströbel P et al. (2014) PMID: 24236644. This antibody has been successfully used in IHC on Pig:

Western Blot: Appr. 60 kDa in transfected HEK293 lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 0.5-1.0 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-AIRE Antikörper (ABIN185643)

Suzuki, Suzuki, Nakai, Sembon, Fuchimoto, Onishi: "Transcriptional and histological analyses of the thymic developmental process in the fetal pig." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 63, Issue 2, pp. 215-25, (2014) (PubMed).

Ströbel, Hartmann, Rosenwald, Kalla, Ott, Friedel, Schalke, Kasahara, Tomaru, Marx: "Corticomedullary differentiation and maturational arrest in thymomas." in: Histopathology, Vol. 64, Issue 4, pp. 557-66, (2014) (PubMed).

Details zu AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Andere Bezeichnung: AIRE

Hintergrund: AIRE, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) , AIRE1, APECED, APS1, APSI, PGA1 , autoimmune regulator (APECED protein), autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy),

Gen-ID: 326

NCBI Accession: NP_000374

Pathways: Chromatin Binding