Amphiphysin Antikörper (Internal Region)

Produktnummer ABIN185628

Dieser Anti-Amphiphysin Antikörper ist ein Ziege Polyklonal Antikörper zur Detektion von Amphiphysin in WB, ELISA und IHC. Geeignet für Human und Maus.

Kurzübersicht für Amphiphysin Antikörper (Internal Region) (ABIN185628)

Target: Amphiphysin (AMPH)

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Amphiphysin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-Amphiphysin Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Amphiphysin / AMPH

Sequenz: KRKDESRISK AE

Spezifität: This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1, NP_647477.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997

Peptide ELISA: antibody detection limit dilution 1:16000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Amphiphysin

Target: Amphiphysin (AMPH)

Andere Bezeichnung: AMPH

Hintergrund: AMPH, amphiphysin, amphiphysin (Stiff-Man syndrome with breast cancer 128 kDa autoantigen) , HGNC:471, AMPH1

Gen-ID: 273, 218038

NCBI Accession: NP_001626, NP_647477