SMAD9 Antikörper (SMAD Family Member 9) (Internal Region)

Details for Product anti-SMAD9 Antibody No. ABIN185609
Antigen
  • SMAD8
  • MADH6
  • MADH9
  • PPH2
  • SMAD8A
  • SMAD8B
  • Madh8
  • Madh9
  • Smad8
  • SMAD family member 9
  • SMAD9
  • smad9
  • Smad9
Epitop
Internal Region
20
10
9
8
4
4
3
3
2
2
2
1
1
1
1
Reaktivität
Human
93
28
19
6
1
1
1
1
1
1
1
1
Wirt
Ziege
65
20
6
4
Klonalität
Polyklonal
Konjugat
Dieser SMAD9 Antikörper ist unkonjugiert
4
3
3
3
3
3
1
1
1
1
1
1
Applikation
ELISA, Enzyme Immunoassay (EIA), Immunohistochemistry (IHC), Western Blotting (WB)
79
49
25
13
6
6
6
5
2
1
Optionen
Verwendungszweck SMAD9
Immunogen Peptide with sequence C-HSEPLMPHNATYPD, from the internal region of the protein sequence according to NP_005896.1.
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN369207.
Sequenz HSEPLMPHNA TYPD
Spezifität This antibody is not expected to cross-react with SMAD1 and SMAD5.
Homologie Human, Mouse, Rat, Dog
Reinigung Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Güteklasse Verified
Plasmids, Primers & others Plasmide, Primers & weitere SMAD9 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung SMAD9 (SMAD9 Antibody Abstract)
Hintergrund SMAD9, SMAD, mothers against DPP homolog 9 (Drosophila)] , HGNC:6774, MADH6, MADH9, SMAD8A, SMAD8B , MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD, mothers against decapentaplegic homolog 9, MAD, mothers against decapentaplegic homolog 9 (Drosophila), Mothers against decapentaplegic, drosophila, homolog of, 9
Gen-ID 4093
NCBI Accession NP_005896
Applikations-hinweise

DS WB Results: Approx 50 kDa band observed in Human Skin lysates (calculated MW of 48.6 kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 μg/mL. Primary incubation was 1 hour.

DS IHC Results: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant SMAD9 captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Minimize freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Aliquot and store at -20 °C.
Bilder des Herstellers
 image for anti-SMAD Family Member 9 (SMAD9) (Internal Region) antibody (ABIN185609) ABIN185609 (0.3µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Pr...
Allgemeine Veröffentlichungen Shi, Massagué: "Mechanisms of TGF-beta signaling from cell membrane to the nucleus." in: Cell, Vol. 113, Issue 6, pp. 685-700, 2003 (PubMed).

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