ERG Antikörper (C-Term)

Produktnummer ABIN185550

Dieses Anti-ERG-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von ERG in WB, ELISA, IF und FACS. Geeignet für Human.

Kurzübersicht für ERG Antikörper (C-Term) (ABIN185550)

Target: ERG (ERG, ETS transcription factor (ERG))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ERG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ERG Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ERG

Sequenz: PNTRLPTSHM PSH

Spezifität: This antibody is expected to recognise all reported isoforms (NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1)

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1, NP_004440.1, NP_001129626.1, NP_001129627.1, NP_001230358.1|.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 55 kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8 kDa according to NP_891548.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Expression of the protein seen in the nucleus and cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ERG

Target: ERG (ERG, ETS transcription factor (ERG))

Andere Bezeichnung: ERG

Hintergrund: ERG, v-ets erythroblastosis virus E26 oncogene like (avian), HGNC:3446, erg-3, p55, transcriptional regulator ERG (transforming protein ERG), v-ets avian erythroblastosis virus E26 oncogene related, v-ets erythroblastosis virus E26 oncogene like, v-ets er

Gen-ID: 2078, 13876, 170909

NCBI Accession: NP_891548, NP_004440, NP_001129626, NP_001129627