HRH2 Antikörper (C-Term)

Produktnummer ABIN185463

Produktdetails anti-HRH2 Antikörper

Target: HRH2 (Histamine Receptor H2 (HRH2))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser HRH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Histamine Receptor H2

Sequenz: QEEKPLKLQV WSGTE

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-QEEKPLKLQVWSGTE, from the C Terminus of the protein sequence according to NP_071640.1, NP_001124527.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 50 kDa band observed in Human Tonsil lysates (calculated MW of 46.9 kDa according to Human NP_001354640.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-HRH2 Antikörper (ABIN185463)

Peng, Zhuang, He, Zhu, Wang: "Histamine excites neurons of the inferior vestibular nucleus in rats by activation of H1 and H2 receptors." in: Neuroscience letters, Vol. 541, pp. 87-92, (2013) (PubMed).

Details zu HRH2

Target: HRH2 (Histamine Receptor H2 (HRH2))

Andere Bezeichnung: HRH2 (HRH2 Produkte)

Synonyme: H2R antikoerper, HH2R antikoerper, HRH2 antikoerper, histamine receptor H2 antikoerper, histamine receptor H2a antikoerper, histamine receptor H 2 antikoerper, HRH2 antikoerper, Hrh2 antikoerper, hrh2a antikoerper

Hintergrund: HRH2, histamine receptor H2, HGNC:5183, H2R, gastric receptor 1, OTTHUMP00000161242

Gen-ID: 3274

NCBI Accession: NP_001124527, NP_001354640