Neuroligin 1 Antikörper (Internal Region)
Kurzübersicht für Neuroligin 1 Antikörper (Internal Region) (ABIN185446)
Target
Alle Neuroligin 1 (NLGN1) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Güteklasse
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Bindungsspezifität
- Internal Region
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Verwendungszweck
- Neuroligin 1
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Sequenz
- TAKQDDPKQQ
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Kreuzreaktivität
- Rind (Kuh), Hund, Human, Maus, Ratte
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Aufreinigung
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogen
- Peptide with sequence C-TAKQDDPKQQ, from the internal region of the protein sequence according to NP_055747.1.
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Isotyp
- IgG
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Applikationshinweise
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Western Blot: Preliminary experiments gave an approx 150 kDa band in Human Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 k
Peptide ELISA: antibody detection limit dilution 1:4000.
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Kommentare
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Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10μg/ml.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Minimize freezing and thawing.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Neuroligin 1 (NLGN1)
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Andere Bezeichnung
- NLGN1
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Hintergrund
- NLGN1, KIAA1070, MGC45115, neuroligin 1, OTTHUMP00000211275, OTTHUMP00000211276
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Gen-ID
- 22871
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NCBI Accession
- NP_055747
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Pathways
- Synaptic Membrane, Synaptic Vesicle Exocytosis
Target
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