PPY Antikörper (Internal Region)

Produktnummer ABIN185420

Der Ziege Polyklonal Anti-PPY-Antikörper wurde für WB, IHC, ELISA, FACS und IF validiert. Er ist geeignet, PPY in Proben von Human und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für PPY Antikörper (Internal Region) (ABIN185420)

Target: PPY (Pancreatic Polypeptide (PPY))

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PPY Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-PPY Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Pancreatic Polypeptide / PPY

Sequenz: TRPRYGKRHK EDT

Kreuzreaktivität: Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TRPRYGKRHKEDT, from the internal region of the protein sequence according to NP_002713.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of rare cells located at the periphery of islets of Langerhans. Recommended concentration: 3 μg/mL. Paraffin embedded Human Pancreas and Intestine. Recommended co

Western Blot: This antibody has been successfully used in WB on Human, PMID: 25445712.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 27300574 and 30687234, and on Rat, PMID: 27472443.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-PPY Antikörper (ABIN185420)

Md Moin, Cory, Ong, Choi, Dhawan, Butler, Butler: "Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes." in: Journal of the Endocrine Society, Vol. 1, Issue 5, pp. 385-395, (2017) (PubMed).

Details zu PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Andere Bezeichnung: PPY

Hintergrund: PPY, pancreatic polypeptide, HGNC:9327, PNP, pancreatic polypeptide Y

Gen-ID: 5539

NCBI Accession: NP_002713

Pathways: Feeding Behaviour