Acetylcholinesterase Antikörper (C-Term)

Produktnummer ABIN185357

Der Ziege Polyklonal Anti-Acetylcholinesterase-Antikörper wurde für WB, ELISA, IF und FACS validiert. Er ist geeignet, Acetylcholinesterase in Proben von Human zu detektieren.

Kurzübersicht für Acetylcholinesterase Antikörper (C-Term) (ABIN185357)

Target: Acetylcholinesterase (AChE)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Acetylcholinesterase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-Acetylcholinesterase Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: ACHE

Sequenz: QFDHYSKQDR CSDL

Spezifität: This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 70 kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: ACHE

Hintergrund: ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase

Gen-ID: 43

NCBI Accession: NP_000656, NP_001269378

Pathways: Skeletal Muscle Fiber Development