Cytochrome B Reductase 1 Antikörper (C-Term)

Produktnummer ABIN185343

Dieses Anti-Cytochrome B Reductase 1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Cytochrome B Reductase 1 in ELISA, WB und IHC. Geeignet für Human. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für Cytochrome B Reductase 1 Antikörper (C-Term) (ABIN185343)

Target: Cytochrome B Reductase 1 (CYBRD1)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Cytochrome B Reductase 1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-Cytochrome B Reductase 1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Cytochrome b reductase 1

Sequenz: CRNLALDEAG QRSTM

Spezifität: This antibody is expected to recognise isoform 1 (NP_079119.3) only.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Schwein

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRNLALDEAGQRSTM, from the C Terminus of the protein sequence according to NP_079119.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Testis shows membranous staining in primarily myofibroblasts of the basement membrane surrounding the seminiferous tubules. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Small Intestine. Rec

Western Blot: Preliminary experiments gave bands at approx 35 kDa and a 26+28 kDa doublet in human colon lysate after 0.1 μg/mL antibody staining. The detected bands were all successfully blocked by with the immunizing peptide. This antibody was successfull

Peptide ELISA: antibody detection limit dilution 1:16000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Cytochrome B Reductase 1 Antikörper (ABIN185343)

Dostalikova-Cimburova, Balusikova, Kratka, Chmelikova, Hejda, Hnanicek, Neubauerova, Vranova, Kovar, Horak: "Role of duodenal iron transporters and hepcidin in patients with alcoholic liver disease." in: Journal of cellular and molecular medicine, Vol. 18, Issue 9, pp. 1840-50, (2014) (PubMed).

Dostalikova-Cimburova, Kratka, Balusikova, Chmelikova, Hejda, Hnanicek, Neubauerova, Vranova, Kovar, Horak: "Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency." in: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1816-26, (2012) (PubMed).

Balusikova, Neubauerova, Dostalikova-Cimburova, Horak, Kovar: "Differing expression of genes involved in non-transferrin iron transport across plasma membrane in various cell types under iron deficiency and excess." in: Molecular and cellular biochemistry, Vol. 321, Issue 1-2, pp. 123-33, (2009) (PubMed).

Details zu Cytochrome B Reductase 1

Target: Cytochrome B Reductase 1 (CYBRD1)

Andere Bezeichnung: CYBRD1

Hintergrund: CYBRD1, DCYTB, FLJ23462, cytochrome b reductase 1, duodenal cytochrome b, FRRS3, ferric-chelate reductase 3

Gen-ID: 79901

NCBI Accession: NP_079119

Pathways: Transition Metal Ion Homeostasis