EHD2 Antikörper (C-Term)

Produktnummer ABIN185287

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch EHD2 in WB, ELISA, IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EHD2 Antikörper (C-Term) (ABIN185287)

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EHD2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-EHD2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: EHD2

Sequenz: CRLVPPSKRR HKGSA

Spezifität: This antibody is expected to recognise EHD1 protein as well as EHD2.

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 65-70 kDa band observed in Human Lung lysates and in lysates of cell lines A549 and HeLa (calculated MW of 61.2 kDa according to NP_055416.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. R

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu EHD2

Target: EHD2 (EH-Domain Containing 2 (EHD2))

Andere Bezeichnung: EHD2

Hintergrund: EHD2, PAST2, EH-domain containing 2, EH domain containing 2, FLJ96617

Molekulargewicht: 61.2kDa

Gen-ID: 30846

NCBI Accession: NP_055416

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development