FOXP3 Antikörper (C-Term)

Produktnummer ABIN185239

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch FOXP3 in FACS, ELISA, IHC und IF. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für FOXP3 Antikörper (C-Term) (ABIN185239)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FOXP3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-FOXP3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: FOXP3 / Scurfin (Mouse)

Sequenz: KRSQRPNKCS NP

Spezifität: Note - anti-mouse companion product of EB05294

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KRSQRPNKCSNP, from the C Terminus of the protein sequence according to NP_473380.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Mouse Spleen. Recommended concentration: 5-6 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Andere Bezeichnung: Foxp3

Hintergrund: Foxp3, forkhead box P3, sf, JM2, scurfin, scurfy, RP23-54C14.1, OTTMUSP00000017952, OTTMUSP00000017961, OTTMUSP00000017962

Gen-ID: 20371, 317382

NCBI Accession: NP_473380

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation