BAF53A and BAF53B (C-Term) Antikörper

Produktnummer ABIN185217

Dieses Anti--Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von in ELISA, WB, FACS und IF. Geeignet für Human, Maus und Ratte.

Kurzübersicht für BAF53A and BAF53B (C-Term) Antikörper (ABIN185217)

Target: BAF53A and BAF53B

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails

Bindungsspezifität: C-Term

Verwendungszweck: BAF53A and BAF53B

Sequenz: YEEGGKQCVE RKCP

Spezifität: This antibody is expected to recognise BAF53A isoforms 1 and 2 and BAF53B, which are almost identical

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence YEEGGKQCVERKCP, from the C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 48 kDa band observed in Rat Skeletal Muscle lysates and approx. 50 kDa in nuclear lysates of cell line NIH3T3 and in preliminary testing of Human Colorectal cancer and Mouse Skeletal Muscle lysate.(predicted MW of 47.4 kDa (Actl6a) acco

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Antigendetails

Target: BAF53A and BAF53B

Andere Bezeichnung: ACTL6A / ACTL6B

Hintergrund: BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B, 53 kDa BRG1-associated factor B, ARPN-BETA, MGC5382 Arp4, INO80K, BAF complex 53 kDa subunit, BAF53, BRG1-associated factor, INO80 com

Gen-ID: 51412, 86

NCBI Accession: NP_004292, NP_817126, NP_057272