Paired Box 3 Antikörper (N-Term)

Produktnummer ABIN185216

Der Ziege Polyklonal Anti-Paired Box 3-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, Paired Box 3 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Paired Box 3 Antikörper (N-Term) (ABIN185216)

Target: Paired Box 3 (PAX3)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Paired Box 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-Paired Box 3 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: PAX3

Sequenz: TTLAGAVPRM M

Spezifität: This antibody is expected to recognize all 8 reported isoforms (NP_852122.1, NP_000429.2, NP_039230.1, NP_852123.1, NP_852124.1, NP_852126.1, NP_852125.1, NP_001120838.1).

Kreuzreaktivität: Rind (Kuh), Human, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TTLAGAVPRMM-C, from the N Terminus of the protein sequence according to NP_000429.2, NP_039230.1, NP_852122.1,.1 NP_852123.1, NP_852124.1, NP_852125.1, NP_852126.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5 μg/mL.

Western Blot: Approx 45 kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2 kDa according to NP_852125.1 and of 44.8 kDa according to NP_852126.1). Recommended concentration: 0.3-1 μg/mL. Primary in

Peptide ELISA: antibody detection limit dilution 1:32000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-Paired Box 3 Antikörper (ABIN185216)

Hsieh, Yao, Lai, Yang: "Transcriptional repression activity of PAX3 is modulated by competition between corepressor KAP1 and heterochromatin protein 1." in: Biochemical and biophysical research communications, Vol. 349, Issue 2, pp. 573-81, (2006) (PubMed).

Details zu Paired Box 3

Target: Paired Box 3 (PAX3)

Andere Bezeichnung: PAX3

Hintergrund: PAX3, WS1, CDHS, HUP2, paired box gene 3 (Waardenburg syndrome 1), paired domain gene 3, paired box homeotic gene 3 (Waardenburg syndrome 1), paired box 3, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, paired box homeotic gene 3, paired domain ge

Gen-ID: 5077, 18505, 114502

NCBI Accession: NP_852122, NP_000429, NP_039230, NP_852123, NP_852124, NP_852126, NP_852125, NP_001120838

Pathways: Sensory Perception of Sound, Tube Formation