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SH3BP1 Antikörper (C-Term)

Verified SH3BP1 Reaktivität: Human WB, ELISA, IF, IHC, ICC Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185183
  • Target Alle SH3BP1 Antikörper anzeigen
    SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))
    Bindungsspezifität
    • 8
    • 6
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 25
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 25
    Ziege
    Klonalität
    • 25
    Polyklonal
    Konjugat
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Dieser SH3BP1 Antikörper ist unkonjugiert
    Applikation
    • 21
    • 18
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
    Verwendungszweck
    SH3BP1
    Sequenz
    PQPRPRSLAS ETN
    Kreuzreaktivität
    Human
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.
    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 μg/mL.

    Western Blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Kommentare

    Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Parrini, Sadou-Dubourgnoux, Aoki, Kunida, Biondini, Hatzoglou, Poullet, Formstecher, Yeaman, Matsuda, Rossé, Camonis: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).

  • Target
    SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))
    Andere Bezeichnung
    SH3BP1 (SH3BP1 Produkte)
    Synonyme
    ARHGAP43 antikoerper, 3BP-1 antikoerper, SH3BP1 antikoerper, SH3 domain binding protein 1 antikoerper, SH3-domain binding protein 1 antikoerper, SH3 domain-binding protein 1 antikoerper, SH3BP1 antikoerper, Sh3bp1 antikoerper, LOC100398528 antikoerper
    Hintergrund
    SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981
    Gen-ID
    23616
    NCBI Accession
    NP_061830
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