SH3BP1 Antikörper (C-Term)

Produktnummer ABIN185183

Der Ziege Polyklonal Anti-SH3BP1-Antikörper wurde für WB, ELISA, IF, IHC und ICC validiert. Er ist geeignet, SH3BP1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für SH3BP1 Antikörper (C-Term) (ABIN185183)

Target: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SH3BP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Güteklasse: Verified

Produktdetails anti-SH3BP1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: SH3BP1

Sequenz: PQPRPRSLAS ETN

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 μg/mL.

Western Blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-SH3BP1 Antikörper (ABIN185183)

Parrini, Sadou-Dubourgnoux, Aoki, Kunida, Biondini, Hatzoglou, Poullet, Formstecher, Yeaman, Matsuda, Rossé, Camonis: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." in: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).

Details zu SH3BP1

Target: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Andere Bezeichnung: SH3BP1

Hintergrund: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981

Gen-ID: 23616

NCBI Accession: NP_061830