VPS35 Antikörper (C-Term)

Produktnummer ABIN185182

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch VPS35 in WB, IHC, IF und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für VPS35 Antikörper (C-Term) (ABIN185182)

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser VPS35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Güteklasse: Verified

Produktdetails anti-VPS35 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: VPS35 / MEM3

Sequenz: SPESEGPIYE GLIL

Spezifität: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 8 μg/mL.

Western Blot: Approx 90 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85 kDa observed in lysates of cell line HEK293 (calculated MW of 91.7 kDa according to Human NP_060676.2, Mouse NP_0

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/vesicles of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-VPS35 Antikörper (ABIN185182)

Klinger, Højland, Jain, Kjolby, Madsen, Svendsen, Olivecrona, Bonifacino, Nielsen: "Polarized trafficking of the sorting receptor SorLA in neurons and MDCK cells." in: The FEBS journal, Vol. 283, Issue 13, pp. 2476-93, (2017) (PubMed).

Lee, Uchida, Emoto, Umeda, Kuge, Taguchi, Arai: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, (2012) (PubMed).

Details zu VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Andere Bezeichnung: VPS35

Hintergrund: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35

Gen-ID: 55737, 65114

NCBI Accession: NP_060676